Transformation method for obtaining marker-free plants and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/11 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2454793

The invention relates to a transformation method for obtaining transgenic plants and plants obtained with said method. The invention provides a method for transforming a plant cell comprising providing a plant cell with a recombinant nucleic acid comprising a T-DNA construct allowing for transfer of said construct into the genome of a plant cell, said construct provided with a foreign nucleic acid that is free of nucleic acid encoding a selective marker.

L'invention se rapporte à un procédé de transformation permettant d'obtenir des plantes transgéniques et plantes ainsi obtenues. L'invention propose un procédé de transformation d'une cellule végétale consistant à doter une cellule végétale d'un acide nucléique recombinant renfermant un gène hybride ADN-T assurant le transfert de ce gène dans le génome d'une cellule végétale, ledit gène hybride étant doté d'un acide nucléique étranger sans acide nucléique codant un marqueur sélectif.

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