Transformation of brassica

A - Human Necessities – 01 – H

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A01H 5/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2522332

The invention involves methods and materials related to the transformation of Brassica by particle bombardment. Specifically, the invention provides methods of preparing non-embryo Brassica tissue such that Brassica cells are capable of being cultured, transformed by particle bombardment, and regenerated into plants. In addition, this invention provides stably transformed Brassica cells as well as their progeny. This invention also provides methods of culturing Brassica tissue with liquid medium such that transformed Brassica cells are identified and regenerated into transformed Brassica plants.

La présente invention a trait à des procédés et des matériaux associés à la transformation de Brassica par bombardement de particules. Notamment, l'invention a trait à des procédés pour la préparation de tissu de Brassica non embryonnaire de sorte que les cellules de Brassica sont capables d'être cultivées, transformées par bombardement de particules et régénérées. En outre l'invention a trait à des cellules de Brassica transformées de manière stable ainsi que leur descendance. L'invention a également trait à des procédés de culture de tissu de Brassica avec un milieu liquide de sorte que les cellules deBrassica transformées sont identifiées et régénérées en plantes de Brassica transformées.

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