C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) C12N 9/02 (2006.01)
Patent
CA 2368841
The present invention is directed to a method for the transformation of a monocot plant comprising exposing explant tissue of said monocot to an Agrobacterium strain under vacuum in the presence of a phenolic compound, said Agrobacterium strain comprising a heterologous gene of interest within a vector. The Agrobacterium is removed from the explant tissue, and an antibiotic against the Agrobacterium is added, and the transformed tissue is plated onto growth medium, grown, and then plated onto selection media. The method may be used with vectors comprising either a vir+ vector, or a super virulent vector.
L'invention porte sur un procédé de transformation de plantes monocotylédones consistant à en exposer les tissus à une souche d'Agrobacterium, sous vide et en présence d'un composé phénolique, ladite souche comprenant un gène hétérologue d'intérêt compris dans un vecteur. On retire ensuite l'Agrobacterium) du tissu, puis on ajoute un antibiotique anti-Agrobacterium, puis on place le tissu transformé sur un milieu de culture, on le fait croître, puis on le place sur un milieu de sélection. Le procédé peut servir avec des vecteurs soit du type vir+ soit du type supervirulent.
Bowley Stephen R.
Devereaux Alissa
Rojas Brenda
Gowling Lafleur Henderson Llp
University Of Guelph
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