A - Human Necessities – 01 – H
Patent
A - Human Necessities
01
H
47/4, 195/1.36
A01H 1/06 (2006.01) A01H 1/02 (2006.01) A01H 4/00 (2006.01) A01H 5/10 (2006.01) C07K 14/425 (2006.01) C12N 15/82 (2006.01)
Patent
CA 1316475
ABSTRACT A method for somatic embryogenesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/l. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed. Methods for transforming somatic tissue from soybean and other Glycine species are also provided. Whole, fertile, transformed plants are obtained.
543663
Adams Thomas R.
Collins Glenn B.
Hartweck Lynn M.
Hildebrand David F.
Lazzeri Paul A.
Adams Thomas R.
Collins Glenn B.
Hartweck Lynn M.
Hildebrand David F.
Lazzeri Paul A.
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