Transformation system in the field of filamentous fungal hosts

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/80 (2006.01) C12N 1/15 (2006.01) C12N 9/16 (2006.01) C12N 9/24 (2006.01) C12N 9/36 (2006.01) C12N 9/38 (2006.01) C12N 9/42 (2006.01) C12N 9/58 (2006.01) C12N 15/55 (2006.01) C12N 15/56 (2006.01) C12N 15/57 (2006.01)

Patent

CA 2345356

A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well as expression- regulating sequences of Chrysosporium genes.

L'invention concerne un nouveau système de transformation dans le domaine des hôtes fongiques filamenteux, utile pour l'expression et la sécrétion de protéines ou de polypeptides hétérologues. L'invention concerne également un procédé permettant de produire de grandes quantités de polypeptide ou de protéine de manière économique. Le système comporte une souche fongique transformée ou transfectée du genre Chrysosporium, plus particulièrement Chrysosporium lucknowense et des mutants ou des dérivés de celle-ci. L'invention concerne également des transformants contenant des séquences codant pour Chrysosporium, ainsi que des séquences de régulation d'expression de gènes de Chrysosporium.

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