Transformation systems for the yeast candida utilis and the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/81 (2006.01) C07K 14/40 (2006.01) C12N 1/19 (2006.01) C12N 9/14 (2006.01) C12N 9/88 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2168037

An reproducible transformation system of a yeast of Candida utilis, a process for expressing a heterologous gene in the transformation system, a vector which can be used in the transformation system and the expression method, and a novel DNA group are disclosed. In particular, the process for expressing a heterologous gene in Candida utilis comprises transforming Candida utilis with a vector comprising a drug-resistance marker, a sequence homologous to the chromosomal DNA of the Candida utilis yeast, and the heterologous gene, culturing the transformant, and isolating the expression product of the heterologous gene.

Lignée transformée reproductible d'une levure Candida utilis; procédé d'expression d'un hétérogène dans ladite lignée; vecteur utilisable dans ladite lignée et son procédé d'expression; et nouveau groupe d'ADN. Ce procédé d'expression d'un hétérogène dans une levure Candida utilis consiste à transformer ladite levure au moyen d'un vecteur contenant un marqueur résistant aux médicaments, une séquence homologue de celle d'un ADN chromosomique d'une levure Candida utilis et un hétérogène, à cultiver la cellule transformée obtenue, et à isoler le produit d'expression de l'hétérogène.

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