C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/84 (2006.01)
Patent
CA 2215763
The invention relates to transformed, embryogenic microspores and progeny thereof characterized by being transformed by Agrobacterium tumefaciens, capable of leading to non-chimeric transformed haploid or doubled haploid embryos that develop into fertile homozygous plants within one generation and containing stably integrated into their genome a foreign DNA, said DNA being characterized in that it comprises at least one gene of interest and at least base pairs within the right border sequence of Agrobacterium T-DNA. The invention furthermore relates to a method for the incorporation of foreign DNA into chromosomes of microspores comprising the following steps: a) infecting of embryogenic microspores with Agrobacteria, which contain plasmids carrying a gene of interest under regulatory control of initiation and termination regions bordered by at least one T-DNA border, b) washing out and killing the Agrobacteria after co-cultivation.
Dormann Mathias
Oelck Michael
Wang Hung-Mei
Agrevo Canada Inc.
Bayer Cropscience Inc.
Smart & Biggar
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