Transformed human pluripotent stem cells and associated methods

Details Transformed human pluripotent stem cells and associated methods Transformed human pluripotent stem cells and associated methods

C - Chemistry, Metallurgy

12

N

C12N 5/0735 (2010.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) A61K 45/00 (2006.01)

Patent

CA 2688760

The present disclosure provides transformed human pluripotent stem cell (t--hPSC). t-hPSCs are not dependent on Oct4 for renewal and survival, however exhibit a sensitivity to reduced levels of the transcription factor Nanog. Also provided are methods of culturing cells for use in a cell-based screening assay comprising placing one or more transformed human pluripotent stem cells into a receptacle and culturing said stem cells in the receptacle to form a monolayer of stem cells without cell overlap. Methods of screening compounds using t-hPSCs are also described.

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