C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 9/06 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12P 7/06 (2006.01) C12P 7/10 (2006.01) C12P 7/18 (2006.01) C12P 7/42 (2006.01) C12P 7/62 (2006.01) C12P 13/04 (2006.01) C12P 13/08 (2006.01)
Patent
CA 2289023
The present invention relates to genetic engineering of production microorganisms used in biotechnology to improve their properties so that they produce useful products more efficiently. The microorganisms express at least one enzyme that causes the functional coupling of the oxidation and reduction of substrates by two pyridine nucleotide-linked dehydrogenase reactions with different specificities for the NAD/NADH and NADP/NADPH coenzyme couples and so facilitates the transfer of electrons between the two coenzyme couples through the said substrates. In particular the invention relates to increasing the yields of products such as ethanol or amino acids from carbon and nitrogen sources such as biomass comprising hexoes, pentoses or their polymers.
La présente invention se rapporte au génie génétique de micro-organismes de production utilisé en biotechnologie pour améliorer les propriétés de ces micro-organismes, afin qu'ils produisent des produits utiles de manière plus efficace. Ces micro-organismes expriment au moins une enzyme qui provoque le couplage fonctionnel de l'oxydation et de la réduction de substrats par deux réactions de déshydrogénase à liaison pyridine-nucléotide présentant différentes spécificités pour les couples de co-enzymes NAD/NADH et NADP/NADPH, et facilite ainsi le transfert d'électrons entre les deux couples de co-enzymes par le biais desdits substrats. En particulier, l'invention consiste à augmenter les rendements en produits tels que l'éthanol ou les acides aminés à partir de sources d'azote et de carbone, telle que la biomasse comprenant des hexoses, des pentoses ou leurs polymères.
Aristidou Aristos
Londesborough John
Penttila Merja
Richard Peter
Ruohonen Laura
Mccarthy Tetrault Llp
Valtion Teknillinen Tutkimuskeskus
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