Transformed strains originated from multidrug efflux protein...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/21 (2006.01) C12P 7/02 (2006.01) C12P 33/06 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2679532

Disclosed is a means for improving the poor conversion efficiency in a conventional bioconversion using a transformant having a gene derived from a heterogeneous organism integrated therein. A microorganism having the deletion of a gene encoding a multidrug efflux protein is used as a host to produce a transformant having a gene derived from a heterogeneous organism integrated therein. By using the transformant in bioconversion, it becomes possible to convert a hydrophobic or amphipathic substrate compound into a desired compound with extremely high efficiency. When an E. coli cell is used as a host, the gene encoding multidrug efflux protein to be deleted may be tolC, acrA, acrB or the like.

L'invention concerne un moyen permettant d'améliorer la faible efficacité de conversion dans une bioconversion classique à l'aide d'un transformant comprenant un gène dérivé d'un organisme hétérogène intégré. On utilise un microorganisme présentant une délétion de gène codant pour une protéine d'efflux multimédicament comme hôte pour produire un transformant comprenant un gène dérivé d'un organisme hétérogène intégré. L'utilisation du transformant en bioconversion permet de convertir un composé de substrat hydrophobe ou amphipathique en un composé désiré à efficacité extrêmement élevé. Lorsqu'on utilise une cellule E. coli comme hôte, le gène codant pour la protéine d'efflux multimédicament à supprimer peut être tolC, acrA, acrB ou analogue.

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