Transformed yeast strains and their use for the production...

Details Transformed yeast strains and their use for the production... Transformed yeast strains and their use for the production...

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 9/02 (2006.01) C12P 7/40 (2006.01) C12P 7/44 (2006.01)

Patent

CA 2293737

The present invention comprises a bioprocess for converting aliphatic compounds, of the form CH3(CH2)nCH3 where n = 4 to 20, to monoterminal and diterminal carboxylates using genetically-engineered organisms. This invention relates to a process for expressing alkane hydroxylating activity in genetically-engineered yeasts Pichia pastoris and Candida maltosa. In addition, the present invention describes a process to produce genetically transformed Candida maltosa strains that have enhanced cytochrome P450 activity and/or gene disruptions in the .beta.-oxidation pathway.

La présente invention concerne un processus biologique permettant de transformer des composés aliphatiques de la forme CH¿3?(CH¿2?)¿n?CH¿3?, dans laquelle n = 4 à 20, en carboxylates à terminaison unique et à terminaison double à l'aide d'organismes génétiquement modifiés. L'invention se rapporte à un processus permettant l'expression d'une activité d'hydroxylation d'alcane dans des levures Pichia pastoris et Candida maltosa génétiquement modifiées. En outre, l'invention concerne un processus permettant de produire des souches de Candida maltosa génétiquement modifiées déployant une activité de cytochrome P-450 améliorée et/ou des disruptions géniques de la voie d'oxydation .beta..

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