C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/1.32
C12P 21/02 (2006.01) C07K 14/495 (2006.01)
Patent
CA 1341508
This invention discloses a new process for preparing a polypeptide containing at least one peptide sequence of the formula I: -Cys-(AA)a-Cys-(AA)b-Cys-(AA)c-Cys-AA-Cys-(AA)d-Cys- ~(I) wherein AA is an amino acid residue selected from Val, Ser, His, Phe, Asn, Lys, Asp, Thr, Gln, Agr, Leu, Glu, Pro, Ala, Gly, Trp and Tyr, and a is 7, b is 4 or 5, c is 10, and d is 8, wherein said polypeptide contains up to about 50 amino acid residues, which comprises sequential coupling of amino acids, in the order given by the polypeptide formula, and the polypeptide contains at least about 50 amino acids. The process comprises synthetically preparing a double stranded DNA sequence which codes for the amino acid sequence of the polypeptide structure, inserting the double stranded DNA into a suitable site in a cloning vehicle or vector to form a recombinant DNA molecule and transforming an appropriate host with the recombinant DNA molecule and transforming an appropriate host with the recombinant DNA molecule to obtain expression of the polypeptide. This is a unique and improved process.
590515
Applied Protein Sciences Llc
Riches Mckenzie & Herbert Llp
Todaro George Joseph
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