Transgenic animal

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) A61K 49/00 (2006.01) C07K 14/47 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2405504

A transgenic rat containing in its genome a nucleotide sequence encoding a Ga subunit protein, which Ga protein subunit is uncoupled from regulation by Regulators of G-Protein Signaling (RGS) proteins, which Gx subunit protein is eventually the dominant-negative G188S mutant of Gax9, which nucleotide sequence is operatively associated with a neuron-specific expression control sequence, wherein the transgenic rat expresses the GA subunit protein in neural cells resulting in extended D-protein coupled receptor signaling mediated by the Ga subunit protein.

L'invention concerne un rat transgénique contenant dans son génome une séquence nucléotidique codant pour une protéine sous-unité G.alpha., cette sous-unité G.alpha. étant insensible à la régulation par les protéines RGS (régulateurs de signalisation de la protéine G), et étant éventuellement le mutant à dominante négative G188S de G.alpha.q. La séquence nucléotidique est associée de manière opératoire à une séquence de commande d'expression spécifique de neurone, le rat transgénique exprimant la protéine sous-unité G.alpha. dans des cellules nerveuses ce qui provoque une signalisation étendue de récepteur couplé à la protéine G modulée par la protéine sous-unité G.alpha..

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