Transgenic animal with enhanced immune response and method...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A01K 67/027 (2006.01) A61P 37/04 (2006.01) C07K 14/705 (2006.01) C07K 16/00 (2006.01) C12N 5/10 (2006.01) C12N 5/16 (2006.01) C12N 15/85 (2006.01) C12P 21/08 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 2670389

The present invention provides a method for producing a transgenic (Tg) non-human animal capable of developing an enhanced humoral immune response against an antigen as compared to a non-transgenic control animal of the same species, comprising introducing into said non-human animal a genetic construct providing for enhanced MHC class I-related neonatal Fc receptor (FcRn) activity. Also provided a Tg non-human animal comprising a genetic construct providing for enhanced FcRn activity, as well as the use of such animal in a non- therapeutical method. Therapeutic genetic constructs and methods are also provided. The present invention further provides methods for producing immunoglobulins.

Cette invention concerne un procédé permettant de produire un animal transgénique (Tg) non humain capable de développer une réponse immunitaire humorale contre un antigène par rapport à un animal de référence non transgénique de la même espèce. Le procédé consiste à introduire dans cet animal non humain une construction génétique fournissant une activité améliorée du récepteur Fc néonatal associé à la classe I du CMH. Cette invention concerne également un animal non humain transgénique comprenant une construction génétique permettant une activité FcRn améliorée, ainsi que l'utilisation d'un tel animal dans un procédé non thérapeutique. L'invention concerne également des méthodes et des constructions génétiques thérapeutiques, ainsi que des procédés permettant de produire des immunoglobulines.

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