Transgenic animals expressing artificial epitope-tagged...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) A01K 67/027 (2006.01) A61K 47/48 (2006.01) A61K 49/00 (2006.01) C07K 14/42 (2006.01) C12N 15/12 (2006.01) C12N 15/85 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2253262

DNA constructs are provided of epitope-tagged proteins or protein fragments which are conveniently purified with immunoaffinity chromatography such as epitope-tagged prion proteins (PrP). Transgenic animals expressing an epitope- tagged protein are provided, including transgenic animals expressing epitope- tagged PrP. Methods for distinguishing between the conformational shapes of a protein and a convenient method for isolating a tagged protein by immunoaffinity chromatographic methods are provided.

L'invention concerne des produits de recombinaison d'ADN comprenant des protéines ou fragments protéiniques marqués par un épitope qui sont aisément purifiés par chromatographie d'affinité immunologique, tels que des protéines prions (PrP) marquées par un épitope. L'invention concerne également des animaux transgéniques exprimant une protéine marquée par un épitope, tels que des animaux transgéniques exprimant des PrP marquées par un épitope. Des procédés permettant de faire une distinction entre les conformations d'une protéine, et un procédé approprié destiné à isoler une protéine marquée par chromatographie d'affinité immunologique sont également décrits.

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