Transgenic animals expressing chimeric antibodies for use in...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) A01K 67/027 (2006.01) C07K 16/00 (2006.01) C07K 16/46 (2006.01) C12N 15/13 (2006.01) A61K 39/395 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2638774

The invention provides transgene constructs for expressing chimeric antibodies, and transgenic non-human host animals carrying such constructs, wherein the chimeric antibodies comprise human variable regions and constant regions of the non-human transgenic host animal. The presence of immunoglobulin constant regions of the host animal allows for generation of improved antibodies in such transgenic host animals. Subsequently, the chimeric antibodies can be readily converted to fully human antibodies using recombinant DNA techniques. Thus, the invention provides compositions and methods for generating human antibodies in which chimeric antibodies raised in vivo in transgenic mice are used as intermediates and then converted to fully human antibodies in vitro.

L'invention concerne des constructions transgéniques destinées à l'expression d'anticorps chimériques, ainsi que des animaux hôtes non humains, transgéniques, les anticorps chimériques comprenant des régions variables humaines et des régions constantes de l'animal hôte transgénique, non humain. La présence de régions constantes d'immunoglobuline de l'animal hôte permet d'obtenir des anticorps améliorés chez ces animaux hôtes transgéniques. Ces anticorps chimériques peuvent ensuite être facilement transformés en anticorps totalement humains par des techniques de recombinaison d'ADN. Ainsi, l'invention concerne des compositions et des méthodes permettant de produire des anticorps humains, dans lesquelles des anticorps chimériques obtenus in vivo chez des souris transgéniques sont utilisés comme produits intermédiaires avant d'être transformés en anticorps totalement humains in vitro.

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