Transgenic animals expressing diabetogenic t-cell receptor...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) C07K 14/72 (2006.01) C07K 14/725 (2006.01) C12N 15/85 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 2230239

This invention relates generally to transgenic animal models useful in testing agents for inhibition of CD4 + and/or CD8+T-cell destruction of pancreatic beta cells. In particular, it relates to transgenic mice which have T-cell receptor .alpha. and/or .beta. chain genes derived from beta cell cytotoxic CD4 + or CD8 + T cells in their genome. The present invention also relates to methods of making T-cell-receptor transgenic animals developing early-onset diabetes, and to methods of evaluating agents for the prevention and treatment of insulin-dependent diabetes mellitus.

L'invention concerne en règle générale les modèles d'animaux transgéniques que l'on utilise pour tester l'aptitude de certains agents à inhiber la destruction par les cellules T CD4 + et/ou CD8 des cellules bêta des îlots de Langerhans du pancréas. Elle concerne en particulier des souris transgéniques ayant dans leur génomes des gènes à chaîne .alpha. et/ou .beta. de récepteurs de cellules T, dérivés de cellules T CD4 + ou CD8 + cytotoxiques pour les cellules bêta. On décrit par ailleurs des procédés de production d'animaux transgéniques à récepteurs des cellules T développant un diabète de type juvénile, ainsi que des procédés relatifs à l'évaluation d'agents pour la prévention et le traitement du diabète sucré diabetes mellitus insulino-dépendant.

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