Transgenic animals incorporating exogenous grf genes

Details Transgenic animals incorporating exogenous grf genes Transgenic animals incorporating exogenous grf genes

C - Chemistry, Metallurgy

12

N

119/75, 195/1.2,

C12N 15/18 (2006.01) A01K 67/027 (2006.01) C12N 15/89 (2006.01)

Patent

CA 1316851

ABSTRACT OF THE DISCLOSURE TRANSGENIC ANIMALS INCORPORATING EXOGENOUS GROWTH HORMONE RELEASING FACTOR (GRF) GENES Transgenic animals are produced having an exogenous GRF-encoding gene inserted in their genome. The preferred gene includes a GRF-encoding sequence portion operably linked to a strong promoter, such as the metallothionein promoter, and the preferred GRF-encoding sequence portion is a fusion of natural gene sequences and cDNA sequences. The GRF- encoding gene is microinjected into fertilized eggs which then incorporate the gene into their genome, and the fertilized eggs are implanted into host mothers. The animals that are born as a result are tested to determine whether the exogenous gene is incorporated and expressed. The transgenic animals grow to larger than normal size. Both male and female transgenic animals are fertile, whereby the animals are useful for breeding to establish fast-growing, enlarged animal lines.

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