Transgenic cancer models in fish

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/00 (2006.01) A01K 67/00 (2006.01) A01K 67/027 (2006.01) C12Q 1/68 (2006.01) G01N 33/00 (2006.01)

Patent

CA 2500967

The present invention provides transgenic fish whose genome has stably- integrated therein an oncogene operably linked to a promoter, Methods of making the transgenic fish and methods for their use are also provided. In one embodiment, the transgenic fish may advantageously be utilized in methods of screening for drugs or agents that modulate oncogene-mediated neoplastic or hyperplastic transformation, or that modulate sensitivity to chemotherapy or radiation therapy. In another embodiment, the transgenic fish may be used methods of identifying mutations that modulate oncogene-mediated neoplastic or hyperplastic transformation, or that modulate sensitivity to chemotherapy or radiation therapy.

L'invention porte sur un poisson transgénique au génome duquel on a intégré de manière stable un oncogène fonctionnellement lié à un promoteur, et sur des méthodes d'élaboration et d'utilisation de poissons transgéniques. Dans une exécution on peut avantageusement utiliser le poisson transgénique dans des méthodes de criblages de médicaments ou d'agents qui modulent les transformations néoplasiques ou hyperplasiques médiées par les oncogènes ou qui modulent la sensibilité à la chimiothérapie ou à la radiothérapie. Dans une autre exécution, on peut utiliser le poisson transgénique dans des méthodes d'identification des mutations qui modulent les transformations néoplasiques ou hyperplasiques médiées par les oncogènes ou qui modulent la sensibilité à la chimiothérapie ou à la radiothérapie.

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