Transgenic chickens

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/877 (2010.01) A01K 67/027 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2595576

The present invention is transgenic chickens obtained from long-term cultures of avian PGCs and techniques to produce and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs are transmitted through the germline to yield transgenic offspring. This invention includes compositions comprising long-term cultures of PGCs that can be genetically modified by gene targeting, that can accept large amounts of foreign DNA and that contribute to the germline of recipient embryos.

L'invention concerne des poulets transgéniques obtenus à partir de cultures à long terme de cellules germinales primordiales (PGC) aviaires, des techniques de production et des oiseaux transgéniques résultant de cultures de PGC prolongées. Dans certains modes de réalisation, ces PGC peuvent être transfectés par des constructions génétiques pour modifier l'ADN du PGC, notamment pour introduire un transgène codant une protéine exogène. Combinés à un embryon aviaire hôte par des procédés connus, ces PGC modifiés sont transmis par la ligne germinale pour obtenir une descendance transgénique. La présente invention porte également sur des compositions contenant des cultures à long terme de PGC, qui peuvent être génétiquement modifiées par ciblage génique et qui participent à la ligne germinale d'embryons récipiendaires.

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