Transgenic, cross-linked hemoglobin

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A01K 67/027 (2006.01) C07K 14/805 (2006.01) C12N 15/85 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2166995

Transgenic, recombinantly cross-linked polymeric human hemoglobins suitable as cell-free blood substitutes have been produced. A plurality of DNA constructs have been designed for efficient expression of modified human hemoglobins in the erythrocytes of the non- human transgenic animals. Substantially pure, non-immunogenic, artificial human hemoglobins are then easily obtained from the erythroid cells of the transgenic animals.

On a obtenu des hémoglobines humaines, polymères, réticulées par recombinaison, transgéniques, appropriées pour servir de substituts du sang exempts de cellules. Une pluralité de produits de recombinaison d'ADN ont été conçus pour exprimer de manière efficace les hémoglobines humaines modifiées dans les érythrocytes des animaux transgéniques. On a ensuite facilement obtenu des hémoglobines humaines artificielles, non immunogènes, pratiquement pures, à partir des érythrocytes des animaux transgéniques.

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