Transgenic fish in the treatment of diabetes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/17 (2006.01) A01K 67/027 (2006.01) A61K 9/66 (2006.01) A61K 35/60 (2006.01) C07K 14/62 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) A61K 38/00 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2191969

This invention relates to transgenic fish containing a humanized insulin gene which has been altered to secrete human insulin and its use in the treatment of diabetes. In the transgenic fish of the present invention, the fish insulin gene has been modified to code for human insulin gene while leaving the regulatory sequences of fish insulin gene intact. Islet transplantation may provide the meticulous glycemia control required in the treatment of diabetes. The islet tissue of the present invention offers an inexpensive and a nearly unlimited supply of human insulin-producing tissue and therefore, may be useful in the treatment of diabetes. In this regard, an improved method of mass isolation of islet tissue is provided by the present invention. The present invention also provides the use of the humanized insulin gene to promote growth in fish.

Poisson transgénique contenant un gène humanisé d'insuline qui a été modifié pour sécréter de l'insuline humaine, et son utilisation dans le traitement du diabète. Dans le poisson transgénique de la présente invention, le gène d'insuline de poisson a été modifié pour coder le gène de l'insuline humaine tout en laissant intactes les séquences régulatrices du gène de l'insuline de poisson. La greffe des îlots de Langerhans peut fournir la régulation précise de la glycémie requise dans le traitement du diabète. Les tissus des îlots de Langerhans de la présente invention constituent une source peu onéreuse et presque illimitée de tissus produisant de l'insuline humaine et partant, peuvent être utiles dans le traitement du diabète. A cet effet, la présente invention concerne un procédé amélioré d'isolation en masse de tissu des îlots de Langerhans. Elle concerne également l'utilisation du gène de l'insuline humanisé pour favoriser la croissance des poissons.

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