Transgenic fish with tissue-specific expression

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/11 (2006.01) A01K 67/027 (2006.01) C07K 14/46 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2293722

Disclosed are transgenic fish, and a method of making transgenic fish, which express transgenes in stable and predictable tissue- or developmentally- specific patterns. The transgenic fish contain transgene constructs with homologous expression sequences. Also disclosed are methods of using such transgenic fish. Such expression of transgenes allows the study of developmental processes, the relationship of cell lineages, the assessment of the effect of specific genes and compounds on the development or maintenance of specific tissues or cell lineages, and the maintenance of lines of fish bearing mutant genes.

L'invention concerne des poissons transgéniques ainsi qu'un procédé de production de ces poissons transgéniques, lesquels expriment des transgènes dans des modèles prévisibles à spécificité tissulaire ou de développement. Les poissons transgéniques contiennent des constructions de transgènes présentant des séquences d'expression homologues. L'invention concerne également des procédés d'utilisation de ces poissons transgéniques. Une telle expression de transgènes permet l'étude de processus de développement, de la relation de lignées cellulaires, de l'évaluation de l'effet de gènes et de composés spécifiques sur le développement ou le maintien de tissus ou de lignées cellulaires spécifiques ainsi que le maintien de lignées de poissons portant des gènes mutants.

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