C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2176109
This invention concerns methods for synthesis and accumulation of fructose polymers in seed, tubers or leaves of transgenic plants by selective expression of a bacterial fructosyltransferase gene. Selective expression includes coordination of timing, tissue specific expression and especially subcellular location. Successful transformants utilize sucrose to synthesize and accumulate fructan in the vacuole of the cell, in established crops, without loss of co-products or concern for yield loss due to degradation during maturation, harvest or storage of the plant. Enhanced fructan production will benefit the fructose sweetener industry and add value to grain used for feed.
L'invention concerne des procédés de synthèse et d'accumulation de polymères de fructose dans des graines, des tubercules ou des feuilles de plantes transgéniques par expression sélective du gène bactérien fructosyltransférase. L'expression sélective comporte la synchronisation, l'expression spécifique au tissu et en particulier la localisation sous-cellulaire. Les agents de transformation efficaces utilisent du saccharose pour synthétiser et accumuler du fructosane dans la vacuole de la cellule, dans des cultures établies, sans perte des coproduits ni baisse du rendement dues à la dégradation pendant la maturation, la récolte ou le stockage de la plante. Une production accrue de fructosane est avantageuse pour l'industrie de fabrication des édulcorants au fructose et elle augmente la valeur des grains utilisés pour l'alimentation.
Caimi Perry Girard
Hershey Howard Paul
Kerr Phillip S.
Bennett Jones Llp
E. I. Du Pont de Nemours And Company
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