Transgenic high tryptophan plants

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/60 (2006.01) A01H 5/00 (2006.01) A23K 1/16 (2006.01) A23K 1/165 (2006.01) A23L 1/00 (2006.01) A23L 1/305 (2006.01) C12N 9/88 (2006.01) C12N 15/00 (2006.01) C12N 15/82 (2006.01) C12P 13/22 (2006.01)

Patent

CA 2709843

A method for altering the tryptophan content in a plant comprising (a) introducing into regenerable cells of a plant a transgene comprising an isolated DNA encoding a monomeric anthranilate synthase comprising an anthranilate synthase a domain and a anthranilate synthase .beta. domain, said monomeric anthranilate synthase further comprising a mutation that increases anthranilate synthase activity or that reduces the sensitivity of the anthranilate synthase to inhibition by tryptophan or an analog thereof, wherein the isolated DNA is operably linked to a promoter functional in a plant cell, to yield transformed plant cells; and (b) regenerating a plant from said transformed plant cells wherein the cells of the plant express the monomeric anthranilate synthase encoded by the isolated DNA in an amount effective to increase the tryptophan content in the plant relative to the tryptophan content in an untransformed plant of the same genetic background.

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