Transgenic microbial polyhydroxyalkanoate producers

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/52 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12N 9/10 (2006.01) C12N 15/53 (2006.01) C12N 15/54 (2006.01) C12N 15/60 (2006.01) C12N 15/90 (2006.01) C12P 7/62 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2339421

Transgenic microbial strains are provided which contain the genes required for PHA formation integrated on the chromosome. The strains are advantageous in PHA production processes, because (1) no plasmids need to be maintained, generally obviating the required use of antibiotics or other stabilizing pressures, and (2) no plasmid loss occurs, thereby stabilizing the number of gene copies per cell throughout the fermentation process, resulting in homogeneous PHA product formation throughout the production process. Genes are integrated using standard techniques, preferably transposon mutagenesis. In a preferred embodiment wherein multiple genes are incorporated, these are incorporated as an operon.

L'invention concerne des souches microbiennes transgéniques qui contiennent les gènes nécessaires à la formation de polyhydroxyalcanoate (PHA) intégré sur chromosome. Les souches sont avantageuses dans des processus de production de PHA, car: (1) elles ne nécessitent l'entretien d'aucun plasmide, ce qui permet d'éviter de manière générale l'utilisation d'antibiotiques ou d'autres moyens de pression de stabilisation; et (2) aucune perte de plasmide ne se produit, ce qui permet de stabiliser le nombre de copies de gène par cellule pendant tout le processus de fermentation, et par conséquent d'assurer la formation d'un produit de PHA homogène pendant tout le processus de production. Des gènes sont intégrés à l'aide de techniques standard, de préférence par mutagenèse par transposon. Dans un mode de réalisation préféré, de multiples gènes sont incorporés comme opéron.

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