Transgenic organisms with lower growth temperature

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 21/02 (2006.01) A01H 5/00 (2006.01) C07K 14/195 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2542576

The present invention in general relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, characterized in that at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts.

La présente invention concerne, en général, la température de croissance d'organismes, notamment, de végétaux et de micro-organismes et la manipulation de la température de culture tolérable. Plus spécifiquement, cette invention a trait à l'expression de protéines hétérologues dans des micro-organismes et, notamment, à l'expression hétérologue de protéines sensibles à la chaleur dans des bactéries, à gram négatif ou à gram positif. Dans un premier aspect, ladite invention a pour objet une méthode de manipulation de cellules et les cellules résultantes, ladite méthode étant caractérisée en ce qu'est exprimé au moins un gène provenant d'un micro-organisme psychrophile codant au moins un chaperon moléculaire ou une chaperonine. De telles cellules sont sélectionnées parmi des cellules eucaryotes cultivées, à savoir, des cellules animales et végétales et des végétaux entiers, des bactéries à gram négatif et à gram positif, des champignons et des levures.

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