Transgenic plants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/02 (2006.01) C12N 9/12 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2732800

The invention provides transgenic plants, specifically tobacco plants, which accumulate the amino acid threonine in their leaves. The biosynthetic pathway leading to production of threonine is tightly regulated, and previous attempts to achieve a transgenic plant which overproduces threonine has compromised the fitness of the plants. This invention has overcome these dif-ficulties and found a method of increasing the level of threonine in plant leaves above the corresponding wild type level without compromising plant fitness, comprising altering plant metabolism to achieve increased production of threonine after the initiation of leaf senescence. This invention involves a genetic construct comprising a senescence specific promoter operably linked to a coding sequence encoding a polypeptide having threonine insensitive aspartate kinase activity.

L'invention porte sur des plantes transgéniques, notamment des plantes de tabac qui accumulent l'acide aminé thréonine dans leurs feuilles. La voie biosynthétique conduisant à la production de thréonine est étroitement régulée, et des tentatives précédentes pour obtenir une plante transgénique qui surproduit la thréonine ont compromis la santé des plantes. L'invention elle concerne un procédé permettant de surmonter ces difficultés. Ce procédé consiste à augmenter le taux de thréonine dans les feuilles de la plante au-dessus du taux correspondant dans le type sauvage sans compromettre la santé des plantes. Ce procédé consiste à: modifier le métabolisme de la plante pour obtenir une production accrue de thréonine après le commencement de la sénescence de feuille. Cette invention fait intervenir un produit de construction génétique qui comporte un promoteur spécifique de sénescence lié de façon fonctionnelle à une séquence de codage, codant pour un polypeptide ayant une activité aspartate kinase insensible à la thréonine.

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