Transgenic plants with tocopherol methyltransferase

Details Transgenic plants with tocopherol methyltransferase Transgenic plants with tocopherol methyltransferase

A - Human Necessities

01

H

A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07H 21/04 (2006.01) C12N 5/04 (2006.01) C12N 9/10 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2298050

Disclosed are gene sequences encoding .gamma.-tocopherol methyltransferases from photosynthetic organisms. The enzyme .gamma.-tocopherol methyltransferase catalyzes the methylation of .gamma.-tocopherol to yield .alpha.-tocopherol, the most bioactive species of tocopherol. .gamma.-Tocopherol methyltransferase is believed to be involved in regulating the relative amounts of the various tocopherols present in photosynthetic organisms. By introducing a genetic construct having a .gamma.-tocopherol methyltransferase coding sequence placed under the control of a plant promoter into a plant, transgenic plants can be made having altered .gamma.-tocopherol methyltransferase expression, to effect dramatic changes in the tocopherol profile of the plant. Transgenic plants can be made that have .alpha.-tocopherol as the predominant tocopherol in their seeds and oils.

L'invention concerne des séquences de gènes codant des .gamma.-tocophérol méthyltransférases provenant d'organismes photosynthétiques. L'enzyme .gamma.-tocophérol méthyltransférase catalyse la méthylation de .alpha.-tocophérol pour produire un .alpha.-tocophérol, l'espèce la plus bioactive de tocophérol. On pense que la .alpha.-tocophérol méthyltransférase est impliquée dans la régulation des quantités relatives des divers tocophérols présents dans des organismes photosynthétiques. L'introduction d'une construction génétique présentant une séquence codant la .alpha.-tocophérol méthyltransférase placée sous la commande d'un promoteur végétal dans une plante permet d'obtenir des plantes transgéniques ayant une expression de .alpha.-tocophérol méthyltransférase modifiée, afin d'obtenir des changements importants dans le profil de tocophérol de la plante. On peut produire des plantes transgéniques présentant un .alpha.-tocophérol en tant que tocophérol prédominant dans leurs graines et leurs huiles.

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