Transgenic reduction of sinapine in crucifera

Details Transgenic reduction of sinapine in crucifera Transgenic reduction of sinapine in crucifera

A - Human Necessities

01

H

A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C07K 16/16 (2006.01) C12N 15/29 (2006.01) C12N 15/62 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2305864

The sinapine content of seeds of Brassica napus, and other crucifera plants, and the resulting seed meal made therefrom, is reduced by transforming cells of the plants to incorporate an expressible exogenous CYP84 monooxygenase enzyme, particularly ferulate 5-hyroxylase (F5H) enzyme, or an antisense equivalent thereof. This allows for the production of a seed meal that is commercially more valuable. Three specific nucleic acid sequences encoding the F5H polypeptide are disclosed, designated BNF5H1, BNF5H2 and BNF5H3, and genetic constructs produced. The antisense suppression of sinapine is preferred, which can reduce the sinapine content of seed meal by up to 40% compared to wild type or vector-only transformed plants.

La teneur en sinapine des graines de Brassica napus et d'autres crucifères ou du tourteau obtenu à partir de ces graines est réduire par la transformation de cellules des plantes de façon à y incorporer une monooxygénase CYP84 exogène, expressible, en particulier la férulate 5-hydroxylase (F5H), ou un équivalent antisens. Ceci permet d'obtenir un tourteau de plus grande valeur commerciale. Trois séquences d'acides nucléiques spécifiques codant le polypeptide F5H, nommées BNF5H1, BNF5H2 et BNF5H3, sont divulguées et des constructions géniques sont produites. La suppression antisens de la sinapine est préférée car elle permet d'abaisser la teneur en sinapine du tourteau jusqu'à 40 %, comparativement à celle de tourteaux obtenus à partir de plantes de type sauvage ou de plantes transformées par un vecteur seulement.

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