Transiently dynamic flow cytometer analysis system

G - Physics – 01 – N

Patent

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G01N 15/10 (2006.01) G01N 15/14 (2006.01)

Patent

CA 2387860

A flow cytometry apparatus (1) and methods to process information incident to particles or cells entrained in a sheath fluid stream allowing assessment, differentiation, assignment, and separation of such particles or cells even at high rates of speed. A first signal processor (18) individually or in combination with at least one additional signal processor (17) for applying compensation transformation on data from a signal (15). Compensation transformation can involve complex operations on data from at least one signal (15) to compensate for one or numerous operating parameters. Compensated parameters can be returned to the first signal processor (18) for providing information upon which to define and differential particles (3) from on another.

Dispositif de cytométrie de flux (1) et procédés servant à traiter l'information concernant des particules ou des cellules entraînées dans un flux liquide sous gaine, ce qui permet d'évaluer, de différencier, d'affecter et de séparer ces particules ou ces cellules, même à des vitesses élevées. Un premier processeur de signaux (18), individuel ou combiné à moins un processeur de signaux supplémentaire (17) sert à appliquer une transformation de correction sur les données provenant d'un signal. Cette transformation de correction peut impliquer des opérations complexes sur les données provenant d'au moins un signal (15) afin de corriger un ou plusieurs paramètres d'exploitation. On peut renvoyer les paramètres corrigés au premier processeur de signaux (18), de manière à lui transmettre une information permettant de définir et de différencier des particules (3) les unes des autres.

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