Transplastomic plants expressing lumen-targeted protein

Details Transplastomic plants expressing lumen-targeted protein Transplastomic plants expressing lumen-targeted protein

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01)

Patent

CA 2653545

The present invention relates to nucleic acid sequences and methods useful in targeting a recombinant protein encoded by a transgene integrated into the chloroplast genome to the thylakoid lumen of chloroplast, whereby said nucleic acid sequences encode bacterial signal peptides. The invention also relates to means and methods for expressing a disulfide-bond containing protein of interest in a transplastomic plant cell.

La présente invention concerne des séquences d'acides nucléiques et des procédés utiles pour le ciblage d'une protéine recombinante, encodée par un transgène intégré dans le génome chloroplastique, sur le lumen des thylakoïdes des chloroplastes, lesdites séquences d'acides nucléiques codant pour des peptides signaux bactériens. L'invention concerne également des moyens et des procédés permettant l'expression d'une protéine d'intérêt comportant une liaison disulfure dans une cellule végétale transplastomique.

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