C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/04 (2006.01) C12N 1/06 (2006.01) C12N 1/10 (2006.01) C12N 1/14 (2006.01) C12N 1/20 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2096194
ABSTRACT OF THE DISCLOSURE An improved transport medium contains a non-ionic detergent, preferably saponin for lysing white blood cells is used in the transport of microbiological samples from mammalian body sites. The improved transport medium contains a lytic agent, preferably saponin to quickly lyse white blood cells present in the sample, which may destroy the microorganisms and their antigenic sites. The preferred lytic agent saponin is present in a concentration from about 0.1 mg to about 10 mg, saponin/ml transport medium, preferably from about 0.5 mg up to about 2 mg saponin per ml transport medium, more preferably about 0.75 to about 2 mg saponin per ml transport medium, and most preferably in a concentration of about 1 mg saponin per ml transport medium. Furthermore, these detergent lytic agents may be combined in the transport medium with phospholipids, suitably L-a Lecithin (phosphatidylcholine) which forms mixed micelles that protect saponin. Other phospholipids include phosphatidylserine, phosphatidylinositol, phosphatidic acid, phosphatidylglycerol, phosphatidylethanolamine and sphingomyelin. If the viability of the microorganisms is to be preserved, the improved transport medium should also include suitable buffers and a carbon source to allow the survival of the microbiological sample. Preferred transport media suitable for use in the present invention include modification (by the addition of saponin) of the following media: (1) Amies Transport Medium Without Charcoal, (2) Amies Transport Medium With Charcoal, and (3) Stuart Transport Medium.
Carski Theodore R.
Sussman Mark L.
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
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