Transposon consisting of a fragment of the r plasmid pcxm82b

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.2, 195/1.2

C12N 15/77 (2006.01) C12N 1/21 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2037431

The invention relates to a transposon consisting of a fragment of the R plasmid pCxM82B from Corynebacterium xerosis DSM 5021, to mobilizable, non-self-transferrable vectors which contain this transposon, and to methods for the mutagenesis of gram-positive bacteria by means of the transfer of these vectors and the generation of auxotrophic mutants.

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