Transposon mediated multiplex sequencing

Details Transposon mediated multiplex sequencing Transposon mediated multiplex sequencing

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2415786

The present invention relates to an automated method of transposon-mediated multiplex sequencing of DNA fragments inserted into a vector. It relates more particularity to an increased efficiency in such automated methods, where the increased efficiency is obtained by screening out before the sequencing those constructs in which the transposon inserted into the vector sequence. This prevents a waste of time and resources in performing reactions sequencing the vector instead of the DNA fragments of interest.

La présente invention concerne un procédé automatisé de séquençage multiplex de fragments d'ADN compris dans un vecteur, induit par des transposons. Plus particulièrement, cette invention concerne l'amélioration de l'efficacité de ces procédés. Cette efficacité étant améliorée grâce à l'élimination par criblage avant le séquençage des constructions présentant un transposon introduit dans la séquence du vecteur. Ceci permet d'empêcher la perte de temps et de ressources dans le déclenchement de réactions entraînant le séquençage du vecteur au lieu de celui des fragments d'ADN intéressants.

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