Treatment of inflammation by inhibition of lipid body formation

G - Physics – 01 – N

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Details

G01N 33/483 (2006.01) A61K 31/4015 (2006.01) A61K 31/405 (2006.01) A61K 31/54 (2006.01) A61K 31/60 (2006.01) A61K 45/00 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2237850

The present invention provides a method for treating a lipid body-mediated condition, such as inflammation or cancer, in a vertebrate by administering to the vertebrate a sufficient amount of an agent which inhibits the formation of lipid bodies in cells involved in inflammation. The agent can inhibit lipid body formation by inhibiting any biological activity necessary for lipid body formation. Another embodiment of the present invention is a method or assay for assessing the ability of a compound to inhibit lipid body formation in cells in vitro. The method comprises the steps of priming the cells for lipid body formation, contacting the cells with the compound under study, and comparing lipid body numbers in the cells with lipid body numbers in primed cells not contacted with the compound of interest. This method can be automated, yielding an efficient high-throughput assay for the efficient and rapid screening of large numbers of potential anti-inflammatory drugs.

Cette invention porte sur une méthode permettant de traiter un état pathologique lié à un corps lipidique, comme une inflammation ou un cancer, chez un vertébré et consistant à lui administrer une quantité efficace d'un agent empêchant la formation de corps lipidiques dans des cellules enflammées. Cet agent est en mesure d'empêcher la formation de corps lipidique en entravant toute activité biologique indispensable à sa formation. Un autre mode de réalisation de cette invention concerne une méthode ou un dosage permettant d'évaluer l'aptitude d'un composé à empêcher la formation de corps lipidiques in vitro. Cette méthode consiste à sensibiliser les cellules propres à former un corps lipidique, à mettre ces cellules en contact avec le composé à étudier et à comparer le nombre de corps lipidiques dans les cellules à celui des cellules sensibilisées n'ayant pas été mises en contact avec le composé en question. Il est possible d'automatiser cette méthode, ce qui permet de disposer d'un dosage à rendement élevé permettant une détermination rapide et efficace d'un grand nombre de médicaments à potentiel anti-inflammatoire.

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