Treatment of staphylococcus infections

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/52 (2006.01) A01K 67/027 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2477596

The present invention relates to an improved approach for the treatment of microbial infections in mammals. Specifically, the invention provides methods and reagents of expressing in mammalian cells, proteins having anti-microbial activity. The invention provides both genes, which have been modified to allow expression and preferably secretion of active protein in desired mammalian cells or tissues, and methods of introducing such modified genes into desired mammalian cells and/or tissues. Most specifically, genes encoding anti- staphylococcal proteins are delivered to mammalian cells and/or tissues by methods of gene delivery, including gene therapy and the production of transgenic animals, for the treatment of mastitis a ruminant animals.

L'invention concerne une approche améliorée du traitement d'infection microbiennes chez les mammifères. Elle concerne spécifiquement des procédés et des réactifs d'expression, dans des cellules de mammifères, de protéines possédant une activité antimicrobienne. L'invention concerne à la fois les gènes, modifiés afin de permettre l'expression et, de préférence, la sécrétion de protéine active dans des cellules ou des tissus d'intérêt de mammifères, ainsi que des procédés d'introduction de tels gènes modifiés dans ces cellules et/ou tissus d'intérêt. Elle concerne, plus spécifiquement, des gènes codant pour des protéines anti-staphylocoques apportés dans des cellules et/ou des tissus de mammifères au moyen de procédés d'apport de gènes, incluant la thérapie génique et la production d'animaux transgéniques, pour le traitement de la mastite chez des ruminants.

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