C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/30 (2006.01) A21D 8/04 (2006.01) C12N 1/15 (2006.01) C12N 15/56 (2006.01) C12P 19/14 (2006.01) D06M 16/00 (2006.01)
Patent
CA 2680794
A maltogenic .alpha.-amylase from Trichoderma reesei (TrAA) and variants thereof in the presence of a glucoamylase are useful in the production of high-glucose syrups from liquefied starch, where the high-glucose syrups produced thereby contain at least about 97% glucose. In this process, TrAA advantageously suppresses the reversion of glucose to malto-oligosaccharides. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.
L'invention concerne une a-amylase maltogène provenant de Trichoderma reesei (TrAA) et des variantes de celle-ci qui, en présence d'une glycoamylase, sont utiles dans la production de sirops à haute teneur en glucose à partir d'amidon liquéfié, les sirops à haute teneur en glucose ainsi produits contenant au moins 97 % de glucose. Dans ce procédé, TrAA supprime avantageusement la réversion du glucose en malto-oligosaccharides. Des hôtes d'expression et des acides nucléiques de codage utiles pour produire TrAA et ses variantes sont également prévus.
Duan Gang
Qian Kathy
Scheffers Martijn
Shetty Jayarama K.
Van Solingen Pieter
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Danisco Us Inc.
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