Tripartite molecular beacons

Details Tripartite molecular beacons Tripartite molecular beacons

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2470287

Tripartite molecular beacons (TMBs), are disclosed that are readily adaptable to high throughput applications. Each tripartite molecular beacon comprises three oligonucleotide components. The first oligonucleotide forms a hairpin stem and loop structure and the second and third oligonucleotides each comprise a sequence complementary to opposite strands of the hairpin stem. The second oligonucleotide has a fluorophore attached thereto and the third oligonucleotide has a quencher attached thereto.

Cette invention concerne des marqueurs moléculaires tripartites pouvant être directement adaptés à des applications à haute capacité. Chacun des marqueurs moléculaires tripartites comprend trois composants oligonucléotides. Le premier oligonucléotide forme une tige en forme de U et une structure en boucle et les deuxième et troisième oligonucléotides comprennent chacun une séquence complémentaire aux brins opposés de la tige en forme de U. Un fluorophore est fixé sur le deuxième oligonucléotide et un extincteur est fixé au troisième oligonucléotide.

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