Tripeptide derivatives and their application in assaying...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/34 (2006.01) C07K 5/06 (2006.01) C07K 5/083 (2006.01) C07K 5/087 (2006.01) C07K 5/093 (2006.01) C07K 5/103 (2006.01) C12Q 1/37 (2006.01) C12Q 1/56 (2006.01)

Patent

CA 1161432

ABSTRACT Tripeptide derivatives having the formula Image I wherein H5 is a chromogenic substituted amino group which is capable of being split off by enzymatic hydrolysis to form a coloured or fluorescent split product H - R5. The tripeptide derivatives of formula I are used for assay- ing certain enzymes, more particularly factor Xa. Enzyme- bearing materials are reacted with the said tripeptide deriv?ives. The quantity of split product H - R5 released by th? enzymatic action of the tripeptide derivative is determined photometrically, spectrophotometrically. fluor- escence-spectrophotometrically or electrochemically. The quantity of released split product H - H is proportional to the quantity of enzyme present in the starting materials.

370232

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