Triple fusion proteins comprising ubiquitin fused between...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) A61K 39/00 (2006.01) A61K 39/12 (2006.01) C07K 14/025 (2006.01) C07K 14/47 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2412084

The present invention relates to novel expression systems, constructs and vectors for use therein, and to the use of the systems to produce recombinant polypeptides suitable for a range of applications, in particular in medicine. The system is characterized by fusion proteins comprising ubiquitin fused between thioredoxin and a polypeptide of interest.

L'invention concerne de nouveaux systèmes d'expression, des constructions et des vecteurs s'utilisant dans ces systèmes, ainsi que l'utilisation de ces systèmes en vue de produire des polypeptides de recombinaison pouvant servir dans une certaine gamme d'applications, notamment en médecine. Ce système est caractérisé en ce que les protéines hybrides contiennent de l'ubiquitine fusionnée entre de la thiorédoxine et un polypeptide d'intérêt.

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