Truncated adamts molecules

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) C07K 16/40 (2006.01) C12N 5/10 (2006.01) C12N 9/50 (2006.01) C12N 15/57 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2561706

The invention provides truncated biologically active ADAMTS polypeptides, particularly those with hyalectenase activity, and more particularly those with aggrecanase activity, that exhibit greater stability and homogeneity and higher expression yields than their full-length counterparts. The invention also provides nucleic acid molecules encoding such truncated biologically active ADAMTS polypeptides and methods for producing the truncated biologically active ADAMTS polypeptides. In addition, the invention provides methods for identifying compounds capable of modulating biologically active ADAMTS polypeptides, particularly those compounds that inhibit aggrecanase activity.

La présente invention a trait à des polypeptides ADAMTS tronqués biologiquement actifs, notamment ceux ayant une activité hyalecténase, et plus particulièrement ceux ayant une activité aggrecanase, présentant une plus grande stabilité et homogénéité et des performances d'expression supérieures à celles de leurs homologues pleine longueur. L'invention a également trait à des molécules d'acides nucléiques codant pour de tels polypeptides ADAMTS tronqués biologiquement actifs et à des procédés pour la production des polypeptides ADAMTS tronqués biologiquement actifs. L'invention a trait en outre à des procédés pour l'identification de composés capables de moduler des polypeptides ADAMTS tronqués biologiquement actifs, notamment ces composés inhibiteurs de l'activité d'aggrecanase.

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