Truncated cellulase compositions

C - Chemistry – Metallurgy – 12 – N

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C12N 15/56 (2006.01) C11D 3/386 (2006.01) C12N 9/42 (2006.01) D06M 16/00 (2006.01) D06P 5/02 (2006.01) D06P 5/13 (2006.01) D06P 5/15 (2006.01)

Patent

CA 2244970

Alkalophilic and thermophilic cellulases having high stability to elevated temperatures and pH have been isolated from an organism of unknown species, which most closely resembles those in the Coldicellulosiruptor genus and which has been called by us, Tok7B.1, These cellulases have been cloned and expressed in a recombinant system, so that they can be produced in quantity. These are particularly useful in treating cellulosic materials including cotton-containing fabrics, as detergent additives, and in aqueous compositions. We also provide genomic DNA which can be used in recombinant expression vectors and expression systems to produce enhanced alkali and/or temperature stability properties in cellulases other than those specifically described.

Des cellulases alcaliphiles et thermophiles, très stables à des températures et à des pH élevés ont été isolées d'un organisme d'espèce inconnue, qui ressemble le plus étroitement aux organismes du genre Coldicellulosiruptor et que nous avons désigné par Tok7B.1. Ces cellulases ont été clonées et exprimées dans un système de recombinant, de sorte qu'elles puissent être produites en quantité. Elles sont particulièrement utiles dans le traitement des matières cellulosiques, y compris les tissus contenant du coton, comme additifs dans les détergents et dans les compositions aqueuses. Nous dévoilons aussi l'ADN génomique qui peut être utilisé dans les vecteurs d'expression et les systèmes d'expression de recombinant pour obtenir des cellulases, autres que celles spécifiquement décrites, plus stables en milieu alcalin et à des températures élevées.

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