Truncated hepatitis c virus ns5 domain and fusion proteins...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/70 (2006.01)

Patent

CA 2566725

The invention provides truncated HCV NS5 polypeptides and fusion proteins comprising the truncated NS5 polypeptides, fused to at least one other HCV epitope derived from another region of the HCV polyprotein. The fusions can be used in methods of stimulating an immune response to HCV, for example a cellular immune response to HCV, such as activating hepatitis C virus (HCV)specific T cells, including CD4+ and CD8+ T cells. The method can be used in model systems to develop HCVspecific immunogenic compositions, as well as to immunize a mammal against HCV.

L'invention concerne des polypeptides NS5 du VHC tronqués et des protéines hybrides comprenant ces polypeptides NS5 tronqués, fusionnés à au moins un autre épitope de VHC dérivé d'une autre région de la polyprotéine de VHC. Ces protéines hybrides peuvent être utilisées dans des méthodes visant à stimuler une réponse immunitaire au VHC, telle qu'une réponse immunitaire cellulaire au VHC, pour activer des lymphocytes T spécifiques du virus de l'hépatite C, y compris des lymphocytes CD4?+¿ et CD8?+¿. Cette méthode peut également être utilisée dans des systèmes modèles destinés à la mise au point de compositions immunogènes spécifiques du VHC, ainsi que pour immuniser un mammifère contre le VHC.

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