Trypsin inhibitor protein from pentaclethra macroloba having...

Details Trypsin inhibitor protein from pentaclethra macroloba having... Trypsin inhibitor protein from pentaclethra macroloba having...

C - Chemistry, Metallurgy

07

K

C07K 14/81 (2006.01) A01N 37/18 (2006.01) C12N 15/82 (2006.01) A01N 65/00 (2006.01)

Patent

CA 2210016

The invention comprises purified trypsin inhibitors extracted from the seeds of Pentaclethra macroloba. The crude extract was found to comprise two molecular weight ranges of 38-35 and 6-9 kDa which contain the active compounds. Within each molecular weight range, the trypsin inhibitors were found to exist in two isoforms. The lower molecular weight inhibitor exhibits surprising stability and activity after heating in aqueous solution at approximately 100 ·C for 30 minutes.

L'invention concerne des inhibiteurs de la trypsine purifiés, extraits des graines de Pentaclethra macroloba. Il a été découvert que l'extrait brut contient des inhibiteurs dont les poids moléculaires couvrent deux plages: 38-35 et 6-9 KDa, lesquels renferment les composés actifs. A l'intérieur de chaque plage, les inhibiteurs de la trypsine existent sous deux isoformes. L'inhibiteur ayant le poids moléculaire le plus bas montre une stabilité et une activité surprenantes après chauffage dans une solution aqueuse à environ 100 ·C pendant 30 minutes.

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