Tumour necrosis factor alpha convertase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/57 (2006.01) A61K 31/16 (2006.01) A61K 31/70 (2006.01) A61K 38/48 (2006.01) A61K 48/00 (2006.01) C07C 323/60 (2006.01) C07D 513/04 (2006.01) C07H 21/00 (2006.01) C12N 9/64 (2006.01) C12N 9/96 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2249985

The present invention relates to tumor necrosis factor alpha (TNF.alpha.), and more specifically to the enzyme TNF.alpha.-convertase (TNF.alpha.-con) that can proteolytically convert TNF.alpha. precursor to mature TNF.alpha.. The present invention provides DNA sequences encoding mammalian TNF.alpha.-con and functional equivalents thereof, recombinant expression vectors comprising said DNA sequences, host cell lines comprising said expression vectors, inhibitors of TNF.alpha.-con, inhibitors modified for use as ligands for affinity purification of TNF.alpha.-con, and methods for treating diseases or conditions resulting from abnormal levels of TNF.alpha. in a mammalian subject.

L'invention porte sur le facteur alpha de nécrose tumorale (TNF alpha ) et plus particulièrement sur l'enzyme TNF alpha -convertase (TNF alpha -con) assurant la conversion protéolytique du précurseur du TNF alpha en TNF alpha à maturité. L'invention porte sur des séquences d'ADN codant pour la TNF alpha -con de mammifère et ses équivalents fonctionnels, sur les vecteurs d'expression de recombinaison comprenant lesdites séquences d'ADN, sur des lignées de cellules hôtes comprenant lesdits vecteurs d'expression, sur des inhibiteurs de la TNF alpha -con, sur des inhibiteurs modifiés pour servir de ligands pour la purification de la TNF alpha -con par affinité, et sur des procédés de traitement de maladies ou d'états pathologiques dus à des taux anormaux de TNF alpha chez des mammifères.

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