Two-hybrid assay that detects hiv-1 reverse transcriptase...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/70 (2006.01) A61K 39/00 (2006.01) C12N 9/12 (2006.01) C12N 15/00 (2006.01) C12P 21/04 (2006.01) C12P 21/06 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2412386

This invention provides methods of determining wheter a compound inhibits HIV- 1 reverse transcriptase. This invention provides methods of determining whether a compound inhibits formation of a complex between a p66 and p51 subunit polypeptides of HIV-1 reverse transcriptase. This invention provides a method of determining whether a compund enhances formation of a complex between a p66 and p51 subunit polypeptides of HIV-1 reverse transcriptase. This invention provides methods of determining whether a compound inhibits formation of a complex between two p66 subunit polypeptides of HIV-1 reverse transcriptase. This invention provides methods of determining whether a compound enhances formation of a complex between two p66 subunit polypeptides of HIV-1 reverse transcriptase.

L'invention concerne des procédés permettant de déterminer si un composé inhibe ou non la transcriptase inverse de HIV-1. Elle concerne des procédés permettant de déterminer si un composé inhibe la formation d'un complexe entre des polypeptides des sous-unités p66 et p51 de la transcriptase inverse de HIV-1, ainsi qu'un procédé permettant de déterminer si un composé renforce la formation d'un complexe entre des polypeptides des sous-unités p66 et p51 de la transcriptase inverse de HIV-1. Elle concerne en outre des procédés permettant de déterminer si un composé inhibe la formation d'un complexe entre deux polypeptides de la sous-unité p66 de la transcriptase inverse de HIV-1 et, enfin, des procédés permettant de déterminer si un composé renforce la formation d'un complexe entre deux polypeptides de la sous-unité p66 de la transcriptase inverse de HIV-1.

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