Two-site immunoassay for an antibody with chemiluminescent...

Details Two-site immunoassay for an antibody with chemiluminescent... Two-site immunoassay for an antibody with chemiluminescent...

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/553 (2006.01) G01N 33/564 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2149340

A method of detecting an antibody in a sample using a labelling compound and comprising the steps of mixing a ligand antigen, antibody or hapten bound to biotin with the sample; an antibody directed against the antibody to be detected bound to paramagnetic particles; and a chemiluminescent acridinium compound bound to avidin or streptavidin to form a solid phase complex; separating the olid phase from the liquid phase; and analysing the separated solid phase for the presence of chemiluminescent complex.

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