Type-1 ribosome-inactivating protein

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) A61K 38/16 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C07K 14/415 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2295189

The invention relates to a novel ribosome-binding protein derived from Bougainvillea spectabilis having a molecular weight of about 26,000 daltons by polyacrylamide gel electrophoresis under reducing and non- reducing conditions, a pI of about 9.0, and further comprising a specified amino-terminal amino acid sequence, as well as to a conjugate of said protein with a targeting ligand, such as an antibody, to form an immunotoxin. The protein and the conjugate are useful in therapy, for example in the control of tumour cells or viruses.

L'invention concerne une nouvelle protéine se fixant au ribosome, possédant un poids moléculaire de 26.000 daltons, obtenue à partir de Bougainvillea spectabilis par électrophorèse sur gel de polyacrylamide dans des conditions réductrices et non réductrices, présentant un pI de 9 et comportant, de plus, une séquence spécifique d'acides aminés à terminaison amino. Elle concerne également un conjugué de ladite protéine avec un ligand de ciblage, tel qu'un anticorps, de manière à produire une immunotoxine. Cette protéine et ce conjugué sont utiles sur un plan thérapeutique afin de lutter, par exemple, contre des cellules tumorales ou des virus.

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