C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/52 (2006.01) C07B 61/00 (2006.01) C12N 9/00 (2006.01) G01N 33/48 (2006.01) G06F 17/30 (2006.01) G06F 17/50 (2006.01)
Patent
CA 2452818
Tyrosyl-tRNA synthetases having a modified amino acid sequence whereby unnatural amino acids can be more efficiently incorporated than original natural amino acids. More specifically, tyrosyl-tRNA synthetases having been modified at the amino acid(s) at the 37- and/or 195- positions. A method of modifying tyrosyl-tRNA synthetase characterized in that the position of an amino acid to be modified is determined based on the 3-D structure of a tyrosyl-AMP and of tyrosyl synthetase complex and an amino acid to which this enzyme binds.
L'invention concerne des tyrosyl-tARN synthases comportant une séquence d'acides aminés modifiée, ces acides aminés non naturels pouvant être plus facilement incorporés que les acides aminés naturels d'origine. L'invention concerne plus précisément des tyrosyl-tARN synthases dont le/les acide(s) aminé(s) a/ont été modifié(s) au niveau des positions 37 et/ou 195. L'invention concerne encore un procédé permettant de modifier une tyrosyl-tARN synthase, ce procédé se caractérisant en ce que la position d'un acide aminé à modifier est déterminée en fonction de la structure tridimensionnelle d'un complexe ANP constitué de tyrosyl synthase et d'un acide aminé auquel se lie ladite enzyme.
Hirao Ichiro
Kiga Daisuke
Sakamoto Kensaku
Yokoyama Shigeyuki
Japan Science And Technology Agency
Ridout & Maybee Llp
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